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TaKaRa
noti restriction sites bolded
Noti Restriction Sites Bolded, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/noti+restriction+sites+bolded/Not+I/us12612397-3089-58-66
Average 97 stars, based on 4281 article reviews
Noti Restriction Sites Bolded, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/noti+restriction+sites+bolded/Not+I/us12612397-3089-58-66
Average 97 stars, based on 4281 article reviews
noti restriction sites bolded - by Bioz Stars,
2026-09
97/100 stars
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Blocking Assay:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA Standard (5834 bps) G Block Sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTG TCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCAC TGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACT TAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGG TGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACA GACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAAT CTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGA AACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTA CAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC. .. FoxM1 A2 (5558 bps) G Block sequence (IDT DNA) (SEQ ID NO: 96) GAATTCGTTTTTGGGGAACAGGTGGTGTTTGGTTACATGAGTAAGTTCTTT AGTGGCGATCTGCGAGATTTTGGTACACCCATCACCAGCTTGTTTAATTTT ATCTTTCTTTGTTTATCAGCGGCCGC FoxM2 BC (6439 bps) G Block sequence (IDT DNA) (SEQ ID NO: 97) GAATTCGGCGGAAGATGAAGCCACTGCTACCACGGGTCAGCTCATACCTGG TACCTATCCAGTTCCCGGTGAACCAGTCACTGGTGTTGCAGCCCTCGGTGA AGGTGCCATTGCCCCTGGCGGCTTCCCTCATGAGCTCAGAGCTTGCCCGCC ATAGCAAGCGAGTCCGCATTGCCCCCAAGGTGCTGCTAGCTGAGGAGGGGA TAGCTCCTCTTTCTTCTGCAGGACCAGGGAAAGAGGAGAAACTCCTGTTTG GAGAAGGGTTTTCTCCTTTGCTTCCAGTTCAGACTATCAAGGAGGAAGAAA TCCAGCCTGGGGAGGAAATGCCACACTTAGCGAGACCCATCAAAGTGGAGA GCCCTCCCTTGGAAGAGTGGCCCTCCCCGGCCCCATCTTTCAAAGAGGAAT CATCTCACTCCTGGGAGGATTCGTCCCAATCTCCCACCCCAAGACCCAAGA AGTCCTACAGTGGGCTTAGGTCCCCAACCCGGTGTGTCTCGGAAATGCTTG TGATTCAACACAGGGAGAGGAGGGAGAGGAGCCGGTCTCGGAGGAAACAGC ATCTACTGCCTCCCTGTGTGGATGAGCCGGAGCTGCTCTTCTCAGAGGGGC CCAGTACTTCCCGCTGGGCCGCAGAGCTCCCGTTCCCAGCAGACTCCTCTG ACCCTGCCTCCCAGCTCAGCTACTCCCAGGAAGTGGGAGGACCTTTTAAGA CACCCATTAAGGAAACGCTGCCCATCTCCTCCACCCCGAGCAAATCTGTCC TCCCCAGAACCCCTGAATCCTGGAGGCTCACGCCCCCAGCCAAAGTAGGGG GACTGGATTTCAGCCCAGTACAAACCTCCCAGGGTGCCTCTGACCCCTTGC CTGACCCCCTGGGGCTGATGGATCTCAGCACCACTCCCTTGCAAAGTGCTC CCCCCCTTGAATCACCGCAAAGGCTCCTCAGTTCAGAACCCTTAGACCTCA TCTCCGTCCCCTTTGGCAACTCTTCTCCCTCAGCGGCCGC MADD Isoform 4 (WT) (5668 bps) G Block sequence (IDT DNA) (SEQ ID NO: 98) GAATTCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTA TTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGT GGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAA CACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGC AATTCTAGTGAGAACCAGCAGTTCCTGCGGCCGC MADD Isoform 3 (Ex16) (5689 bps) G Block sequence (IDT DNA) (SEQ ID NO: 99) GAATTCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTAT CGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAA GGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGG GCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAA AGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAAGCGG CCGC MAPTau Full length (4R) (5654 bps) G Block sequence (IDT DNA) (SEQ ID NO: 100) GAATTCTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGAC CTGAAGAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAG CCGGGAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAAC GTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGC GGCAGTGTGCAAGCGGCCGC MAPTau Truncation (3R) (5644 bps) G Block sequence (IDT DNA) (SEQ ID NO: 101) GAATTCTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG GAGGCGGGAAGGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGA CCTCCAAGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCC AGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGA AGGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 108) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGT GTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTC ACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAA ACTTAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAA GTGGTGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 109) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTAC AGACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCA ATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGT GGAAACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTT TTTACAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Sequencing:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA Standard (5834 bps) G Block Sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTG TCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCAC TGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACT TAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGG TGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACA GACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAAT CTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGA AACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTA CAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC. .. FoxM1 A2 (5558 bps) G Block sequence (IDT DNA) (SEQ ID NO: 96) GAATTCGTTTTTGGGGAACAGGTGGTGTTTGGTTACATGAGTAAGTTCTTT AGTGGCGATCTGCGAGATTTTGGTACACCCATCACCAGCTTGTTTAATTTT ATCTTTCTTTGTTTATCAGCGGCCGC FoxM2 BC (6439 bps) G Block sequence (IDT DNA) (SEQ ID NO: 97) GAATTCGGCGGAAGATGAAGCCACTGCTACCACGGGTCAGCTCATACCTGG TACCTATCCAGTTCCCGGTGAACCAGTCACTGGTGTTGCAGCCCTCGGTGA AGGTGCCATTGCCCCTGGCGGCTTCCCTCATGAGCTCAGAGCTTGCCCGCC ATAGCAAGCGAGTCCGCATTGCCCCCAAGGTGCTGCTAGCTGAGGAGGGGA TAGCTCCTCTTTCTTCTGCAGGACCAGGGAAAGAGGAGAAACTCCTGTTTG GAGAAGGGTTTTCTCCTTTGCTTCCAGTTCAGACTATCAAGGAGGAAGAAA TCCAGCCTGGGGAGGAAATGCCACACTTAGCGAGACCCATCAAAGTGGAGA GCCCTCCCTTGGAAGAGTGGCCCTCCCCGGCCCCATCTTTCAAAGAGGAAT CATCTCACTCCTGGGAGGATTCGTCCCAATCTCCCACCCCAAGACCCAAGA AGTCCTACAGTGGGCTTAGGTCCCCAACCCGGTGTGTCTCGGAAATGCTTG TGATTCAACACAGGGAGAGGAGGGAGAGGAGCCGGTCTCGGAGGAAACAGC ATCTACTGCCTCCCTGTGTGGATGAGCCGGAGCTGCTCTTCTCAGAGGGGC CCAGTACTTCCCGCTGGGCCGCAGAGCTCCCGTTCCCAGCAGACTCCTCTG ACCCTGCCTCCCAGCTCAGCTACTCCCAGGAAGTGGGAGGACCTTTTAAGA CACCCATTAAGGAAACGCTGCCCATCTCCTCCACCCCGAGCAAATCTGTCC TCCCCAGAACCCCTGAATCCTGGAGGCTCACGCCCCCAGCCAAAGTAGGGG GACTGGATTTCAGCCCAGTACAAACCTCCCAGGGTGCCTCTGACCCCTTGC CTGACCCCCTGGGGCTGATGGATCTCAGCACCACTCCCTTGCAAAGTGCTC CCCCCCTTGAATCACCGCAAAGGCTCCTCAGTTCAGAACCCTTAGACCTCA TCTCCGTCCCCTTTGGCAACTCTTCTCCCTCAGCGGCCGC MADD Isoform 4 (WT) (5668 bps) G Block sequence (IDT DNA) (SEQ ID NO: 98) GAATTCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTA TTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGT GGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAA CACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGC AATTCTAGTGAGAACCAGCAGTTCCTGCGGCCGC MADD Isoform 3 (Ex16) (5689 bps) G Block sequence (IDT DNA) (SEQ ID NO: 99) GAATTCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTAT CGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAA GGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGG GCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAA AGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAAGCGG CCGC MAPTau Full length (4R) (5654 bps) G Block sequence (IDT DNA) (SEQ ID NO: 100) GAATTCTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGAC CTGAAGAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAG CCGGGAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAAC GTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGC GGCAGTGTGCAAGCGGCCGC MAPTau Truncation (3R) (5644 bps) G Block sequence (IDT DNA) (SEQ ID NO: 101) GAATTCTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG GAGGCGGGAAGGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGA CCTCCAAGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCC AGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGA AGGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 108) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGT GTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTC ACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAA ACTTAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAA GTGGTGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 109) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTAC AGACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCA ATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGT GGAAACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTT TTTACAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Expressing:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA Standard (5834 bps) G Block Sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTG TCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCAC TGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACT TAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGG TGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACA GACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAAT CTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGA AACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTA CAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC. .. FoxM1 A2 (5558 bps) G Block sequence (IDT DNA) (SEQ ID NO: 96) GAATTCGTTTTTGGGGAACAGGTGGTGTTTGGTTACATGAGTAAGTTCTTT AGTGGCGATCTGCGAGATTTTGGTACACCCATCACCAGCTTGTTTAATTTT ATCTTTCTTTGTTTATCAGCGGCCGC FoxM2 BC (6439 bps) G Block sequence (IDT DNA) (SEQ ID NO: 97) GAATTCGGCGGAAGATGAAGCCACTGCTACCACGGGTCAGCTCATACCTGG TACCTATCCAGTTCCCGGTGAACCAGTCACTGGTGTTGCAGCCCTCGGTGA AGGTGCCATTGCCCCTGGCGGCTTCCCTCATGAGCTCAGAGCTTGCCCGCC ATAGCAAGCGAGTCCGCATTGCCCCCAAGGTGCTGCTAGCTGAGGAGGGGA TAGCTCCTCTTTCTTCTGCAGGACCAGGGAAAGAGGAGAAACTCCTGTTTG GAGAAGGGTTTTCTCCTTTGCTTCCAGTTCAGACTATCAAGGAGGAAGAAA TCCAGCCTGGGGAGGAAATGCCACACTTAGCGAGACCCATCAAAGTGGAGA GCCCTCCCTTGGAAGAGTGGCCCTCCCCGGCCCCATCTTTCAAAGAGGAAT CATCTCACTCCTGGGAGGATTCGTCCCAATCTCCCACCCCAAGACCCAAGA AGTCCTACAGTGGGCTTAGGTCCCCAACCCGGTGTGTCTCGGAAATGCTTG TGATTCAACACAGGGAGAGGAGGGAGAGGAGCCGGTCTCGGAGGAAACAGC ATCTACTGCCTCCCTGTGTGGATGAGCCGGAGCTGCTCTTCTCAGAGGGGC CCAGTACTTCCCGCTGGGCCGCAGAGCTCCCGTTCCCAGCAGACTCCTCTG ACCCTGCCTCCCAGCTCAGCTACTCCCAGGAAGTGGGAGGACCTTTTAAGA CACCCATTAAGGAAACGCTGCCCATCTCCTCCACCCCGAGCAAATCTGTCC TCCCCAGAACCCCTGAATCCTGGAGGCTCACGCCCCCAGCCAAAGTAGGGG GACTGGATTTCAGCCCAGTACAAACCTCCCAGGGTGCCTCTGACCCCTTGC CTGACCCCCTGGGGCTGATGGATCTCAGCACCACTCCCTTGCAAAGTGCTC CCCCCCTTGAATCACCGCAAAGGCTCCTCAGTTCAGAACCCTTAGACCTCA TCTCCGTCCCCTTTGGCAACTCTTCTCCCTCAGCGGCCGC MADD Isoform 4 (WT) (5668 bps) G Block sequence (IDT DNA) (SEQ ID NO: 98) GAATTCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTA TTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGT GGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAA CACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGC AATTCTAGTGAGAACCAGCAGTTCCTGCGGCCGC MADD Isoform 3 (Ex16) (5689 bps) G Block sequence (IDT DNA) (SEQ ID NO: 99) GAATTCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTAT CGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAA GGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGG GCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAA AGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAAGCGG CCGC MAPTau Full length (4R) (5654 bps) G Block sequence (IDT DNA) (SEQ ID NO: 100) GAATTCTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGAC CTGAAGAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAG CCGGGAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAAC GTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGC GGCAGTGTGCAAGCGGCCGC MAPTau Truncation (3R) (5644 bps) G Block sequence (IDT DNA) (SEQ ID NO: 101) GAATTCTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG GAGGCGGGAAGGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGA CCTCCAAGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCC AGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGA AGGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 108) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGT GTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTC ACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAA ACTTAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAA GTGGTGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 109) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTAC AGACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCA ATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGT GGAAACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTT TTTACAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Plasmid Preparation:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA Standard (5834 bps) G Block Sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTG TCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCAC TGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACT TAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGG TGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACA GACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAAT CTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGA AACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTA CAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC. .. FoxM1 A2 (5558 bps) G Block sequence (IDT DNA) (SEQ ID NO: 96) GAATTCGTTTTTGGGGAACAGGTGGTGTTTGGTTACATGAGTAAGTTCTTT AGTGGCGATCTGCGAGATTTTGGTACACCCATCACCAGCTTGTTTAATTTT ATCTTTCTTTGTTTATCAGCGGCCGC FoxM2 BC (6439 bps) G Block sequence (IDT DNA) (SEQ ID NO: 97) GAATTCGGCGGAAGATGAAGCCACTGCTACCACGGGTCAGCTCATACCTGG TACCTATCCAGTTCCCGGTGAACCAGTCACTGGTGTTGCAGCCCTCGGTGA AGGTGCCATTGCCCCTGGCGGCTTCCCTCATGAGCTCAGAGCTTGCCCGCC ATAGCAAGCGAGTCCGCATTGCCCCCAAGGTGCTGCTAGCTGAGGAGGGGA TAGCTCCTCTTTCTTCTGCAGGACCAGGGAAAGAGGAGAAACTCCTGTTTG GAGAAGGGTTTTCTCCTTTGCTTCCAGTTCAGACTATCAAGGAGGAAGAAA TCCAGCCTGGGGAGGAAATGCCACACTTAGCGAGACCCATCAAAGTGGAGA GCCCTCCCTTGGAAGAGTGGCCCTCCCCGGCCCCATCTTTCAAAGAGGAAT CATCTCACTCCTGGGAGGATTCGTCCCAATCTCCCACCCCAAGACCCAAGA AGTCCTACAGTGGGCTTAGGTCCCCAACCCGGTGTGTCTCGGAAATGCTTG TGATTCAACACAGGGAGAGGAGGGAGAGGAGCCGGTCTCGGAGGAAACAGC ATCTACTGCCTCCCTGTGTGGATGAGCCGGAGCTGCTCTTCTCAGAGGGGC CCAGTACTTCCCGCTGGGCCGCAGAGCTCCCGTTCCCAGCAGACTCCTCTG ACCCTGCCTCCCAGCTCAGCTACTCCCAGGAAGTGGGAGGACCTTTTAAGA CACCCATTAAGGAAACGCTGCCCATCTCCTCCACCCCGAGCAAATCTGTCC TCCCCAGAACCCCTGAATCCTGGAGGCTCACGCCCCCAGCCAAAGTAGGGG GACTGGATTTCAGCCCAGTACAAACCTCCCAGGGTGCCTCTGACCCCTTGC CTGACCCCCTGGGGCTGATGGATCTCAGCACCACTCCCTTGCAAAGTGCTC CCCCCCTTGAATCACCGCAAAGGCTCCTCAGTTCAGAACCCTTAGACCTCA TCTCCGTCCCCTTTGGCAACTCTTCTCCCTCAGCGGCCGC MADD Isoform 4 (WT) (5668 bps) G Block sequence (IDT DNA) (SEQ ID NO: 98) GAATTCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTA TTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGT GGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAA CACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGC AATTCTAGTGAGAACCAGCAGTTCCTGCGGCCGC MADD Isoform 3 (Ex16) (5689 bps) G Block sequence (IDT DNA) (SEQ ID NO: 99) GAATTCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTAT CGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAA GGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGG GCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAA AGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAAGCGG CCGC MAPTau Full length (4R) (5654 bps) G Block sequence (IDT DNA) (SEQ ID NO: 100) GAATTCTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGAC CTGAAGAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAG CCGGGAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAAC GTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGC GGCAGTGTGCAAGCGGCCGC MAPTau Truncation (3R) (5644 bps) G Block sequence (IDT DNA) (SEQ ID NO: 101) GAATTCTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG GAGGCGGGAAGGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGA CCTCCAAGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCC AGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGA AGGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 108) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGT GTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTC ACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAA ACTTAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAA GTGGTGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 109) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTAC AGACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCA ATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGT GGAAACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTT TTTACAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Cloning:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA Standard (5834 bps) G Block Sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTG TCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCAC TGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACT TAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGG TGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACA GACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAAT CTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGA AACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTA CAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC. .. FoxM1 A2 (5558 bps) G Block sequence (IDT DNA) (SEQ ID NO: 96) GAATTCGTTTTTGGGGAACAGGTGGTGTTTGGTTACATGAGTAAGTTCTTT AGTGGCGATCTGCGAGATTTTGGTACACCCATCACCAGCTTGTTTAATTTT ATCTTTCTTTGTTTATCAGCGGCCGC FoxM2 BC (6439 bps) G Block sequence (IDT DNA) (SEQ ID NO: 97) GAATTCGGCGGAAGATGAAGCCACTGCTACCACGGGTCAGCTCATACCTGG TACCTATCCAGTTCCCGGTGAACCAGTCACTGGTGTTGCAGCCCTCGGTGA AGGTGCCATTGCCCCTGGCGGCTTCCCTCATGAGCTCAGAGCTTGCCCGCC ATAGCAAGCGAGTCCGCATTGCCCCCAAGGTGCTGCTAGCTGAGGAGGGGA TAGCTCCTCTTTCTTCTGCAGGACCAGGGAAAGAGGAGAAACTCCTGTTTG GAGAAGGGTTTTCTCCTTTGCTTCCAGTTCAGACTATCAAGGAGGAAGAAA TCCAGCCTGGGGAGGAAATGCCACACTTAGCGAGACCCATCAAAGTGGAGA GCCCTCCCTTGGAAGAGTGGCCCTCCCCGGCCCCATCTTTCAAAGAGGAAT CATCTCACTCCTGGGAGGATTCGTCCCAATCTCCCACCCCAAGACCCAAGA AGTCCTACAGTGGGCTTAGGTCCCCAACCCGGTGTGTCTCGGAAATGCTTG TGATTCAACACAGGGAGAGGAGGGAGAGGAGCCGGTCTCGGAGGAAACAGC ATCTACTGCCTCCCTGTGTGGATGAGCCGGAGCTGCTCTTCTCAGAGGGGC CCAGTACTTCCCGCTGGGCCGCAGAGCTCCCGTTCCCAGCAGACTCCTCTG ACCCTGCCTCCCAGCTCAGCTACTCCCAGGAAGTGGGAGGACCTTTTAAGA CACCCATTAAGGAAACGCTGCCCATCTCCTCCACCCCGAGCAAATCTGTCC TCCCCAGAACCCCTGAATCCTGGAGGCTCACGCCCCCAGCCAAAGTAGGGG GACTGGATTTCAGCCCAGTACAAACCTCCCAGGGTGCCTCTGACCCCTTGC CTGACCCCCTGGGGCTGATGGATCTCAGCACCACTCCCTTGCAAAGTGCTC CCCCCCTTGAATCACCGCAAAGGCTCCTCAGTTCAGAACCCTTAGACCTCA TCTCCGTCCCCTTTGGCAACTCTTCTCCCTCAGCGGCCGC MADD Isoform 4 (WT) (5668 bps) G Block sequence (IDT DNA) (SEQ ID NO: 98) GAATTCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTA TTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGT GGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAA CACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGC AATTCTAGTGAGAACCAGCAGTTCCTGCGGCCGC MADD Isoform 3 (Ex16) (5689 bps) G Block sequence (IDT DNA) (SEQ ID NO: 99) GAATTCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTAT CGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAA GGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGG GCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAA AGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAAGCGG CCGC MAPTau Full length (4R) (5654 bps) G Block sequence (IDT DNA) (SEQ ID NO: 100) GAATTCTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGAC CTGAAGAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAG CCGGGAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAAC GTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGC GGCAGTGTGCAAGCGGCCGC MAPTau Truncation (3R) (5644 bps) G Block sequence (IDT DNA) (SEQ ID NO: 101) GAATTCTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG GAGGCGGGAAGGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGA CCTCCAAGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCC AGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGA AGGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAA CCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCC GTCGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACA AGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA AGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGT CCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATAC GGGTCCTGGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGT TCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 108) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGT GTCCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTC ACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAA ACTTAGAAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAA GTGGTGCGGCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 109) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTAC AGACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCA ATCTCAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGT GGAAACCTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTT TTTACAGAATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA) (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAAC CGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGT CGACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGG TCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGA TTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTG TCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCT ATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCT GGCATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGG CCGC SMN2FL standard G Block sequence (IDT DNA) are used SMN2Δ7 standard G Block sequence (IDT DNA) are used The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Clone Assay:Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard Construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and Article Title: Methods and compositions for modulating splicing Article Snippet: .. Standard construction PPIA standard (5834 bps) G Block sequence (IDT DNA (SEQ ID NO: 95) GAATTCGGCCAGGCTCGTGCCGTTTTGCAGACGCCACCGCCGAGGAAAACC GTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCG ACGGCGAGCCCTTGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCC CAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAAAGGATTTG GTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGG GTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGG AGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCT TGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCGCGGCCGC IKBKAP WT (5639 bps) (SEQ ID NO: 112) GAATTCCTTCATTTAAAACATTACAGGCCGGCCTGAGCAGCAATCATGTGT CCCATGGGGAAGTTCTGCGGAAAGTGGAGAGGGGTTCACGGATTGTCACTG TTGTGCCCCAGGACACAAAGCTTGTATTACAGATGCCAAGGGGAAACTTAG AAGTTGTTCATCATCGAGCCCTGGTTTTAGCTCAGATTCGGAAGTGGTGCG GCCGC IKBKAP MU (5645 bps) (SEQ ID NO: 113) GAATTCCGGATTGTCACTGTTGTGCCCCAGGACACAAAGCTTGTATTACAG ACTTATGTTTAAAGAGGCATTTGAATGCATGAGAAAGCTGAGAATCAATCT CAATCTGATTTATGATCATAACCCTAAGGTGTTTCTTGGAAATGTGGAAAC CTTCATTAAACAGATAGATTCTGTGAATCATATTAACTTGTTTTTTACAGA ATTGCGGCCGC The G Blocks are inserted into the pCI-neo mammalian expression vector (Promega) at the EcoRI and |